Regulatory

Part:BBa_K1179069:Design

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-18)


CAG promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable.

Source

CAG is a strong synthetic constitutive promoter to drive high levels of expression in mammalian vectors. Sincere thanks to the Weiss Lab for contributing this entry vector to the 2013 MIT iGEM team

References